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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P02931




SWISS-2DPAGE:  P02931


P02931


General information about the entry
View entry in simple text format
Entry nameOMPF_ECOLI
Primary accession numberP02931
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 2)
General Annotations were last modified on May 19, 2011 (version 11)
Name and origin of the protein
DescriptionRecName: Full=Outer membrane protein F; AltName: Full=Outer membrane protein 1A; AltName: Full=Outer membrane protein B; AltName: Full=Outer membrane protein IA; AltName: Full=Porin OmpF; Flags: Precursor;.
Gene nameName=ompF
Synonyms=cmlB, coa, cry, tolF
OrderedLocusNames=b0929, JW0912
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: HOMOTRIMER
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=4.60; Mw=36242

MAPPING (identification):
GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=4.56; Mw=37316  [identification data]
pI=4.64; Mw=37000  [identification data]
pI=4.71; Mw=37000  [identification data]

EXPRESSION:
decrease after benzoic acid treatment [3].

MAPPING (identification):
Tandem mass spectrometry [3].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
Cross-references
2DBase-EcoliP02931; OMPF_ECOLI.
ECO2DBASEB036.0; 6TH EDITION.
UniProtKB/Swiss-ProtP02931; OMPF_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameOMPF_ECOLI
Primary accession numberP02931
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on October 19, 2011 (version 135)
Name and origin of the protein
DescriptionRecName: Full=Outer membrane protein F; AltName: Full=Outer membrane protein 1A; AltName: Full=Outer membrane protein B; AltName: Full=Outer membrane protein IA; AltName: Full=Porin OmpF; Flags: Precursor;
Gene nameName=ompF
Synonyms=cmlB, coa, cry, tolF
OrderedLocusNames=b0929, JW0912
Encoded onName=ompF; Synonyms=cmlB, coa, cry, tolF; OrderedLocusNames=b0929, JW0912
Keywords3D-structure; Cell membrane; Cell outer membrane; Complete proteome; Direct protein sequencing; Ion transport; Membrane; Porin; Reference proteome; Signal; Transmembrane; Transmembrane beta strand; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ01655; AAA24244.1; -; Genomic_DNA
EMBLU00096; AAC74015.1; -; Genomic_DNA
EMBLAP009048; BAA35675.1; -; Genomic_DNA
PIRA93449; MMECF; .
RefSeqNP_415449.1; NC_000913.2; .
PDB1BT9; X-ray; 3.00 A; A=23-362
PDB1GFM; X-ray; 3.50 A; A=23-362
PDB1GFN; X-ray; 3.10 A; A=23-362
PDB1GFO; X-ray; 3.30 A; A=23-362
PDB1GFP; X-ray; 2.70 A; A=23-362
PDB1GFQ; X-ray; 2.80 A; A=23-362
PDB1HXT; X-ray; 2.40 A; A=23-362
PDB1HXU; X-ray; 3.00 A; A=23-362
PDB1HXX; X-ray; 2.20 A; A=23-362
PDB1MPF; X-ray; 3.00 A; A=23-362
PDB1OPF; X-ray; 3.20 A; A/B/C/D/E/F=23-362
PDB2OMF; X-ray; 2.40 A; A=23-362
PDB2ZFG; X-ray; 1.59 A; A=23-362
PDB2ZLD; X-ray; 3.00 A; A/B=23-362
PDB3FYX; X-ray; 3.40 A; A=23-362
PDB3HW9; X-ray; 2.61 A; A/B=1-362
PDB3HWB; X-ray; 3.00 A; A/B=1-362
PDB3K19; X-ray; 3.79 A; A/B/C/D/E/F/G/H/I/J/K/L=23-362
PDB3K1B; X-ray; 4.39 A; A/B/C/D=23-362
PDBsum1BT9; -; .
PDBsum1GFM; -; .
PDBsum1GFN; -; .
PDBsum1GFO; -; .
PDBsum1GFP; -; .
PDBsum1GFQ; -; .
PDBsum1HXT; -; .
PDBsum1HXU; -; .
PDBsum1HXX; -; .
PDBsum1MPF; -; .
PDBsum1OPF; -; .
PDBsum2OMF; -; .
PDBsum2ZFG; -; .
PDBsum2ZLD; -; .
PDBsum3FYX; -; .
PDBsum3HW9; -; .
PDBsum3HWB; -; .
PDBsum3K19; -; .
PDBsum3K1B; -; .
ProteinModelPortalP02931; -; .
SMRP02931; 23-362; .
DIPDIP-10398N; -; .
IntActP02931; 2; .
TCDB1.B.1.1.1; general bacterial porin (GBP) family; .
SWISS-2DPAGEP02931; -; .
2DBase-EcoliP02931; -; .
ECO2DBASEB036.0; 6TH EDITION; .
PRIDEP02931; -; .
EnsemblBacteriaEBESCT00000004263; EBESCP00000004263; EBESCG00000003475; .
EnsemblBacteriaEBESCT00000016555; EBESCP00000015846; EBESCG00000015615; .
GeneID945554; -; .
GenomeReviewsAP009048_GR; JW0912; .
GenomeReviewsU00096_GR; b0929; .
KEGGecj:JW0912; -; .
KEGGeco:b0929; -; .
EchoBASEEB0665; -; .
EcoGeneEG10671; ompF; .
eggNOGCOG3203; -; .
GeneTreeEBGT00050000008697; -; .
HOGENOMHBG673855; -; .
OMACIALVLP; -; .
ProtClustDBPRK10002; -; .
BioCycEcoCyc:EG10671-MONOMER; -; .
GenevestigatorP02931; -; .
GOGO:0009279; C:cell outer membrane; IDA:EcoCyc; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-KW; .
GOGO:0046930; C:pore complex; IDA:EcoCyc; .
GOGO:0042912; F:colicin transmembrane transporter activity; IMP:EcoCyc; .
GOGO:0015238; F:drug transmembrane transporter activity; IMP:EcoCyc; .
GOGO:0015075; F:ion transmembrane transporter activity; TAS:EcoCyc; .
GOGO:0015288; F:porin activity; IMP:EcoliWiki; .
InterProIPR023614; Porin_dom; .
InterProIPR001897; Porin_gammaproteobac; .
InterProIPR001702; Porin_Gram-ve; .
InterProIPR013793; Porin_Gram-ve_CS; .
Gene3DG3DSA:2.40.160.10; Porin_Gram-ve; 1; .
PfamPF00267; Porin_1; 1; .
PRINTSPR00183; ECOLIPORIN; .
PRINTSPR00182; ECOLNEIPORIN; .
PROSITEPS00576; GRAM_NEG_PORIN; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server