General information about the entry |
View entry in simple text format |
Entry name | CH60_ECOLI |
Primary accession number | P0A6F5 |
Secondary accession number(s) | P06139 |
integrated into SWISS-2DPAGE on | August 1, 1995 (release 2) |
2D Annotations were last modified on | March 31, 2004 (version 5) |
General Annotations were last modified on | May 19, 2011 (version 14) |
Name and origin of the protein |
Description | RecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName: Full=Protein Cpn60;. |
Gene name | Name=groL Synonyms=groEL, mopA OrderedLocusNames=b4143, JW4103
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Annotated species | Escherichia coli [TaxID: 562] |
Taxonomy | Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. |
References |
[1] |
MAPPING ON GEL MEDLINE=96314059; PubMed=8740179; [NCBI, Expasy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F. ''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):547-555(1996)
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[2] |
MAPPING ON GEL Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C. ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'' (IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
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[3] |
PROTEIN EXPRESSION MEDLINE=94042879; PubMed=8226654; [NCBI, Expasy, EBI, Israel, Japan]
Gage D.J., Neidhardt F.C. ''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';'' J. Bacteriol. 175(1):7105-7108(1993)
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[4] |
PROTEIN EXPRESSION MEDLINE=87083376; PubMed=3539918; [NCBI, Expasy, EBI, Israel, Japan]
Vanbogelen R.A., Kelley P.M., Neidhardt F.C. ''''''Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli'';'';'' J. Bacteriol. 169(1):26-32(1987)
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[5] |
MAPPING ON GEL PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C. ''''''New perspectives in the Escherichia coli proteome investigation'';'';'' Proteomics 1(1):409-423(2001)
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[6] |
MAPPING ON GEL PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S. ''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';'' Proteomics 2(1):1682-1698(2002)
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Comments |
- SUBUNIT: OLIGOMER OF 14 SUBUNITS COMPOSED OF TWO STACKED RINGS OF 7 SUBUNITS
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2D PAGE maps for identified proteins
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How to interpret a protein
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ECOLI {Escherichia coli} Escherichia coli
map experimental info protein estimated location
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ECOLI
MAP LOCATIONS:
EXPRESSION: INCREASED FOLLOWING EXPOSURE TO DNP [3]; WAS INDUCED BY TEMPERATURE SHIFTS, ETHANOL, NALIDIXIC ACID AND PUROMYCIN [4].
MAPPING (identification): AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
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ECOLI4-5 {Escherichia coli(4-5)} Escherichia coli
map experimental info protein estimated location
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ECOLI4-5
MAP LOCATIONS:
MAPPING (identification): Peptide mass fingerprinting [5]; SPOTS 1HGK, 1HGU: GEL MATCHING WITH MASTER ECOLI4.5-5.5 [5].
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ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)} Escherichia coli
map experimental info protein estimated location
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ECOLI4.5-5.5
MAP LOCATIONS:
MAPPING (identification):
Peptide mass fingerprinting [5]; SPOT 2D-0014V3: MICROSEQUENCING (XLRG) [5]. |
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ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)} Escherichia coli
map experimental info protein estimated location
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ECOLI-DIGE4.5-6.5
MAP LOCATIONS:
MAPPING (identification):
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Copyright |
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). |
Cross-references |
2DBase-Ecoli | P0A6F5; CH60_ECOLI. |
ECO2DBASE | B056.5; 6TH EDITION. |
UniProtKB/Swiss-Prot | P0A6F5; CH60_ECOLI. |
2D PAGE maps for identified proteins
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- How to interpret a protein map
- You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
- Warning 1: the displayed region reflects
an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information.
It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
- Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
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