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Protein list for SWISS-2DPAGE ECOLI4.5-5.5 
Escherichia coli(4.5-5.5) 


(map ID: 7d86abb6-faad-450f-bcfa-a21bb11949eb)
Release: 19.2 -- 09 November 2011

Sample Preparation / Gel Related Documents


Protein list for ecoli4.5-5.5   97 identified spots / 65 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods References
aceA RecName: Full=Isocitrate lyase; Short=ICL; Short=Isocitrase; Short=Isocitratase; EC=4.1.3.1; P0A9G6
(ACEA_ECOLI)
2D-00156C
2D-0014YM
2D-001564
2D-0014Z4
4.98
5.19
5.01
5.12
33'669
44'136
33'806
44'136
{PMF}
{PMF}
{PMF}
{PMF}
1
1
1
1
acnB RecName: Full=Aconitate hydratase 2; Short=Aconitase; EC=4.2.1.3; AltName: Full=2-methylisocitrate dehydratase; EC=4.2.1.99; AltName: Full=Citrate hydro-lyase; P36683
(ACON2_ECOLI)
2D-0014R3
5.24
92'389
{PMF}
1
adk RecName: Full=Adenylate kinase; Short=AK; EC=2.7.4.3; AltName: Full=ATP-AMP transphosphorylase; P69441
(KAD_ECOLI)
2D-0015GO
4.99
10'074
{Mi}
1
ahpC RecName: Full=Alkyl hydroperoxide reductase subunit C; EC=1.11.1.15; AltName: Full=Alkyl hydroperoxide reductase protein C22; AltName: Full=Peroxiredoxin; AltName: Full=SCRP-23; AltName: Full=Sulfate starvation-induced protein 8; Short=SSI8; AltName: Full=Thioredoxin peroxidase; P0AE08
(AHPC_ECOLI)
2D-0015CW
2D-0015D5
5.03
5.08
22'398
21'653
{PMF}
{Mi, PMF}
1
1
aldA RecName: Full=Lactaldehyde dehydrogenase; EC=1.2.1.22; AltName: Full=Aldehyde dehydrogenase A; AltName: Full=Glycolaldehyde dehydrogenase; EC=1.2.1.21; P25553
(ALDA_ECOLI)
2D-00153A
2D-001508
5.53
5.29
38'941
42'560
{PMF}
{PMF}
1
1
argG RecName: Full=Argininosuccinate synthase; EC=6.3.4.5; AltName: Full=Citrulline--aspartate ligase; P0A6E4
(ASSY_ECOLI)
2D-0014XO
5.28
46'220
{PMF}
1
argT RecName: Full=Lysine-arginine-ornithine-binding periplasmic protein; Short=LAO-binding protein; Flags: Precursor; P09551
(ARGT_ECOLI)
2D-0015AC
5.17
26'913
{Mi,PMF}
1
aroD RecName: Full=3-dehydroquinate dehydratase; Short=3-dehydroquinase; EC=4.2.1.10; AltName: Full=Type I DHQase; P05194
(AROD_ECOLI)
2D-0015A3
5.31
27'372
{PMF}
1
asnS RecName: Full=Asparaginyl-tRNA synthetase; EC=6.1.1.22; AltName: Full=Asparagine--tRNA ligase; Short=AsnRS; P0A8M0
(SYN_ECOLI)
2D-001533
5.01
39'019
{PMF}
1
atpC RecName: Full=ATP synthase epsilon chain; AltName: Full=ATP synthase F1 sector epsilon subunit; AltName: Full=F-ATPase epsilon subunit; P0A6E6
(ATPE_ECOLI)
2D-0015GA
5.34
10'459
{PMF}
1
atpD RecName: Full=ATP synthase subunit beta; EC=3.6.3.14; AltName: Full=ATP synthase F1 sector subunit beta; AltName: Full=F-ATPase subunit beta; P0ABB4
(ATPB_ECOLI)
2D-0014ZV
2D-0014XV
4.96
5.00
42'905
45'494
{Mi,PMF}
{Mi,PMF}
1
1
clpS RecName: Full=ATP-dependent Clp protease adapter protein ClpS; P0A8Q6
(CLPS_ECOLI)
2D-0015G6
5.40
10'724
{PMF}
1
crr RecName: Full=Glucose-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=EIIA-Glc; AltName: Full=EIII-Glc; AltName: Full=PTS system glucose-specific EIIA component; P69783
(PTGA_ECOLI)
2D-0015DT
4.94
18'465
{Mi}
1
cysK RecName: Full=Cysteine synthase A; Short=CSase A; EC=2.5.1.47; AltName: Full=O-acetylserine (thiol)-lyase A; Short=OAS-TL A; AltName: Full=O-acetylserine sulfhydrylase A; AltName: Full=Sulfate starvation-induced protein 5; Short=SSI5; P0ABK5
(CYSK_ECOLI)
2D-00159M
2D-00156W
2D-00158P
2D-00159J
4.95
5.32
5.06
5.04
28'177
32'863
29'467
28'314
{PMF}
{PMF}
{Mi, PMF}
{PMF}
1
1
1
1
cysM RecName: Full=Cysteine synthase B; Short=CSase B; EC=2.5.1.47; AltName: Full=O-acetylserine (thiol)-lyase B; Short=OAS-TL B; AltName: Full=O-acetylserine sulfhydrylase B; P16703
(CYSM_ECOLI)
2D-001HEM
5.47
31'435
{PMF}
1
dnaK RecName: Full=Chaperone protein DnaK; AltName: Full=HSP70; AltName: Full=Heat shock 70 kDa protein; AltName: Full=Heat shock protein 70; P0A6Y8
(DNAK_ECOLI)
2D-0014VD
2D-0014TQ
2D-0014TV
4.95
4.96
4.97
53'154
60'487
60'010
{PMF}
{Mi, PMF}
{PMF}
1
1
1
engD RecName: Full=GTP-dependent nucleic acid-binding protein engD; P0ABU2
(ENGD_ECOLI)
2D-001505
5.02
42'560
{PMF}
1
eno RecName: Full=Enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=2-phosphoglycerate dehydratase; P0A6P9
(ENO_ECOLI)
2D-00157J
2D-0014Y3
2D-001523
5.16
5.46
5.20
31'562
45'494
40'139
{PMF}
{Mi, PMF}
{PMF}
1
1
1
fabD RecName: Full=Malonyl CoA-acyl carrier protein transacylase; Short=MCT; EC=2.3.1.39; P0AAI9
(FABD_ECOLI)
2D-001573
5.03
32'271
{PMF}
1
fliD RecName: Full=Flagellar hook-associated protein 2; Short=HAP2; AltName: Full=Filament cap protein; AltName: Full=Flagellar cap protein; P24216
(FLID_ECOLI)
2D-0014ZV
4.96
42'905
{Mi}
1
fliY RecName: Full=Cystine-binding periplasmic protein; Short=CBP; AltName: Full=Protein fliY; AltName: Full=Sulfate starvation-induced protein 7; Short=SSI7; Flags: Precursor; P0AEM9
(FLIY_ECOLI)
2D-00159C
2D-0015AN
5.23
5.19
28'658
26'525
{Mi}
{PMF}
1
1
fusA RecName: Full=Elongation factor G; Short=EF-G; P0A6M8
(EFG_ECOLI)
2D-0014R2
5.21
91'354
{PMF}
1
gapA RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A; Short=GAPDH-A; EC=1.2.1.12; P0A9B2
(G3P1_ECOLI)
2D-0015HJ
2D-0015G5
5.32
5.03
9'173
10'690
{Mi}
{Mi}
1
1
gpmI RecName: Full=2,3-bisphosphoglycerate-independent phosphoglycerate mutase; Short=BPG-independent PGAM; Short=Phosphoglyceromutase; Short=iPGM; EC=5.4.2.1; P37689
(GPMI_ECOLI)
2D-0014VM
5.15
52'457
{PMF}
1
gpt RecName: Full=Xanthine phosphoribosyltransferase; EC=2.4.2.22; AltName: Full=Xanthine-guanine phosphoribosyltransferase; Short=XGPRT; P0A9M5
(XGPT_ECOLI)
2D-0015EY
5.44
14'028
{PMF}
1
grcA RecName: Full=Autonomous glycyl radical cofactor; P68066
(GRCA_ECOLI)
2D-0015H1
2D-0015FR
2D-0015GW
2D-0015H7
5.23
5.09
5.05
5.21
9'795
11'594
9'918
9'583
{PMF}
{PMF}
{Mi}
{Mi}
1
1
1
1
groL RecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName: Full=Protein Cpn60; P0A6F5
(CH60_ECOLI)
2D-0014V3
2D-0014UQ
4.96
4.99
54'287
55'445
{Mi, PMF}
{PMF}
1
1
groS RecName: Full=10 kDa chaperonin; AltName: Full=GroES protein; AltName: Full=Protein Cpn10; P0A6F9
(CH10_ECOLI)
2D-0015FD
5.23
13'055
{PMF}
1
grxD RecName: Full=Glutaredoxin-4; Short=Grx4; AltName: Full=Monothiol glutaredoxin; P0AC69
(GLRX4_ECOLI)
2D-0015GQ
4.96
10'012
{PMF}
1
hdeB RecName: Full=Protein hdeB; AltName: Full=10K-L protein; Flags: Precursor; P0AET2
(HDEB_ECOLI)
2D-0015H5
5.00
9'673
{Mi}
1
hisJ RecName: Full=Histidine-binding periplasmic protein; Short=HBP; Flags: Precursor; P0AEU0
(HISJ_ECOLI)
2D-00159H
5.14
28'451
{Mi,PMF}
1
hpt RecName: Full=Hypoxanthine phosphoribosyltransferase; Short=HPRT; EC=2.4.2.8; P0A9M2
(HPRT_ECOLI)
2D-0015FG
5.38
12'380
{PMF}
1
icd RecName: Full=Isocitrate dehydrogenase [NADP]; Short=IDH; EC=1.1.1.42; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase; P08200
(IDH_ECOLI)
2D-0014YW
5.15
43'958
{PMF}
1
ilvB RecName: Full=Acetolactate synthase isozyme 1 large subunit; Short=AHAS-I; EC=2.2.1.6; AltName: Full=Acetohydroxy-acid synthase I large subunit; Short=ALS-I; P08142
(ILVB_ECOLI)
2D-0014UM
5.22
56'329
{PMF}
1
katG RecName: Full=Catalase-peroxidase; Short=CP; EC=1.11.1.21; AltName: Full=Hydroperoxidase I; Short=HPI; AltName: Full=Peroxidase/catalase; P13029
(KATG_ECOLI)
2D-0014RB
5.16
88'816
{PMF}
1
livJ RecName: Full=Leu/Ile/Val-binding protein; Short=LIV-BP; Flags: Precursor; P0AD96
(LIVJ_ECOLI)
2D-0015E7
2D-00151X
5.21
5.31
16'972
40'301
{Mi,PMF}
{Mi,PMF}
1
1
livK RecName: Full=Leucine-specific-binding protein; Short=L-BP; Short=LS-BP; Flags: Precursor; P04816
(LIVK_ECOLI)
2D-00158X
2D-00152C
5.56
5.08
29'053
39'816
{PMF}
{Mi, PMF}
1
1
luxS RecName: Full=S-ribosylhomocysteine lyase; EC=4.4.1.21; AltName: Full=AI-2 synthesis protein; AltName: Full=Autoinducer-2 production protein LuxS; P45578
(LUXS_ECOLI)
2D-0015DS
2D-0015DR
5.07
5.20
18'639
18'697
{PMF}
{Mi, PMF}
1
1
malE RecName: Full=Maltose-binding periplasmic protein; AltName: Full=MBP; AltName: Full=MMBP; AltName: Full=Maltodextrin-binding protein; Flags: Precursor; P0AEX9
(MALE_ECOLI)
2D-00152X
2D-00153H
5.22
5.24
39'098
38'549
{Mi, PMF}
{Mi}
1
1
manX RecName: Full=PTS system mannose-specific EIIAB component; AltName: Full=EIIAB-Man; Includes: RecName: Full=Mannose-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=EIII-Man; AltName: Full=PTS system mannose-specific EIIA component; Includes: RecName: Full=Mannose-specific phosphotransferase enzyme IIB component; EC=2.7.1.69; AltName: Full=PTS system mannose-specific EIIB component; P69797
(PTNAB_ECOLI)
2D-0015AV
5.17
26'207
{PMF}
1
metK RecName: Full=S-adenosylmethionine synthase; Short=AdoMet synthase; EC=2.5.1.6; AltName: Full=MAT; AltName: Full=Methionine adenosyltransferase; P0A817
(METK_ECOLI)
2D-00158A
2D-0014ZS
5.00
5.13
30'312
43'079
{PMF}
{PMF}
1
1
mglB RecName: Full=D-galactose-binding periplasmic protein; Short=GBP; AltName: Full=D-galactose/ D-glucose-binding protein; Short=GGBP; Flags: Precursor; P0AEE5
(DGAL_ECOLI)
2D-00157N
5.19
31'245
{PMF}
1
minD RecName: Full=Septum site-determining protein MinD; AltName: Full=Cell division inhibitor MinD; P0AEZ3
(MIND_ECOLI)
2D-00158K
5.28
29'766
{PMF}
1
nfnB RecName: Full=Oxygen-insensitive NAD(P)H nitroreductase; EC=1.-.-.-; AltName: Full=Dihydropteridine reductase; EC=1.5.1.34; AltName: Full=FMN-dependent nitroreductase; P38489
(NFNB_ECOLI)
2D-0015EU
2D-001HEN
5.09
5.55
14'160
25'092
{PMF}
{PMF}
1
1
pgk RecName: Full=Phosphoglycerate kinase; EC=2.7.2.3; P0A799
(PGK_ECOLI)
2D-00152G
2D-00151K
5.14
5.15
39'816
40'464
{Mi}
{Mi, PMF}
1
1
pnp RecName: Full=Polyribonucleotide nucleotidyltransferase; EC=2.7.7.8; AltName: Full=Polynucleotide phosphorylase; Short=PNPase; P05055
(PNP_ECOLI)
2D-0014RF
5.13
87'327
{PMF}
1
potD RecName: Full=Spermidine/putrescine-binding periplasmic protein; Short=SPBP; Flags: Precursor; P0AFK9
(POTD_ECOLI)
2D-00155G
4.96
35'058
{Mi,PMF}
1
ppa RecName: Full=Inorganic pyrophosphatase; EC=3.6.1.1; AltName: Full=Pyrophosphate phospho-hydrolase; Short=PPase; P0A7A9
(IPYR_ECOLI)
2D-0015D7
5.06
21'653
{Mi,PMF}
1
proS RecName: Full=Prolyl-tRNA synthetase; EC=6.1.1.15; AltName: Full=Global RNA synthesis factor; AltName: Full=Proline--tRNA ligase; Short=ProRS; P16659
(SYP_ECOLI)
2D-0014US
5.06
55'738
{PMF}
1
ribH RecName: Full=6,7-dimethyl-8-ribityllumazine synthase; Short=DMRL synthase; Short=Lumazine synthase; EC=2.5.1.9; AltName: Full=Riboflavin synthase beta chain; P61714
(RISB_ECOLI)
2D-0015FE
5.19
12'934
{Mi,PMF}
1
rplI RecName: Full=50S ribosomal protein L9; P0A7R1
(RL9_ECOLI)
2D-0015HM
5.10
8'919
{PMF}
1
rpoA RecName: Full=DNA-directed RNA polymerase subunit alpha; Short=RNAP subunit alpha; EC=2.7.7.6; AltName: Full=RNA polymerase subunit alpha; AltName: Full=Transcriptase subunit alpha; P0A7Z4
(RPOA_ECOLI)
2D-00152B
2D-001527
5.06
5.03
39'655
39'816
{PMF}
{PMF}
1
1
rpsA RecName: Full=30S ribosomal protein S1; P0AG67
(RS1_ECOLI)
2D-0014SJ
2D-00150A
4.99
4.98
64'780
42'474
{PMF}
{PMF}
1
1
serC RecName: Full=Phosphoserine aminotransferase; EC=2.6.1.52; AltName: Full=Phosphohydroxythreonine aminotransferase; Short=PSAT; P23721
(SERC_ECOLI)
2D-00152W
2D-00156Y
5.47
5.18
39'256
32'731
{PMF}
{PMF}
1
1
talB RecName: Full=Transaldolase B; EC=2.2.1.2; P0A870
(TALB_ECOLI)
2D-00155M
5.08
34'775
{PMF}
1
tig RecName: Full=Trigger factor; Short=TF; P0A850
(TIG_ECOLI)
2D-0014X1
2D-0014WR
2D-0014XC
4.97
4.93
4.95
48'212
48'981
47'081
{PMF}
{PMF}
{PMF}
1
1
1
tpx RecName: Full=Thiol peroxidase; EC=1.11.1.-; AltName: Full=Scavengase P20; P0A862
(TPX_ECOLI)
2D-0015DZ
5.00
17'510
{PMF}
1
trpA RecName: Full=Tryptophan synthase alpha chain; EC=4.2.1.20; P0A877
(TRPA_ECOLI)
2D-00159R
2D-001598
5.37
5.38
27'906
28'936
{PMF}
{PMF}
1
1
trxA RecName: Full=Thioredoxin-1; Short=Trx-1; P0AA25
(THIO_ECOLI)
2D-0015H6
4.95
9'553
{PMF}
1
tsf RecName: Full=Elongation factor Ts; Short=EF-Ts; P0A6P1
(EFTS_ECOLI)
2D-00156V
5.22
32'533
{PMF}
1
tufB RecName: Full=Elongation factor Tu 2; Short=EF-Tu 2; AltName: Full=P-43; P0CE48
(EFTU2_ECOLI)
2D-0014Y6
2D-00158F
5.58
5.14
45'311
30'129
{PMF}
{PMF}
1
1
uspA RecName: Full=Universal stress protein A; P0AED0
(USPA_ECOLI)
2D-0015FP
5.20
11'704
{Mi,PMF}
1
yceI RecName: Full=Protein yceI; Flags: Precursor; P0A8X2
(YCEI_ECOLI)
2D-0015DI
5.20
19'716
{Mi}
1
yciI RecName: Full=Protein yciI; P0AB55
(YCII_ECOLI)
2D-0015H8
5.29
9'553
{PMF}
1
znuA RecName: Full=High-affinity zinc uptake system protein znuA; Flags: Precursor; P39172
(ZNUA_ECOLI)
2D-00155Y
5.58
34'287
{PMF}
1
Mapping legends: { Mi } => 'Microsequencing', { PMF } => 'Peptide mass fingerprinting'



Cited references

# PubMed/Medline Authors Location
[1] 11680886 Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.; Proteomics 1(1):409-423(2001).
''''''New perspectives in the Escherichia coli proteome investigation'';'';'';


Gel experimental information

Information Type Data
Map name ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Dimension 2-D
Related documents Sample Preparation / Gel Related Documents
Strain Comment [species] W3110 [Escherichia coli / TaxID:562]
pI start: 4.50 - end: 5.50
Mw start: 10000 - end: 200000
Number of detected spots 1027
Number of identified spots 97
Number of identified proteins 65
Reference Bibliographic reference
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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server