Attention: World-2DPAGE is no longer maintained.

RecName: Full=Aconitate hydratase 2; Short=Aconitase; EC=4.2.1.3; AltName: Full=2-methylisocitrate dehydratase; EC=4.2.1.99; AltName: Full=Citrate hydro-lyase;

P36683
(ACON2_ECOLI)

2D-0014R3

5.24

92'389

{PMF}

adk

RecName: Full=Adenylate kinase; Short=AK; EC=2.7.4.3; AltName: Full=ATP-AMP transphosphorylase;

P69441
(KAD_ECOLI)

2D-0015GO

4.99

10'074

{Mi}

ahpC

RecName: Full=Alkyl hydroperoxide reductase subunit C; EC=1.11.1.15; AltName: Full=Alkyl hydroperoxide reductase protein C22; AltName: Full=Peroxiredoxin; AltName: Full=SCRP-23; AltName: Full=Sulfate starvation-induced protein 8; Short=SSI8; AltName: Full=Thioredoxin peroxidase;

P0AE08
(AHPC_ECOLI)

2D-0015CW

2D-0015D5

5.03

5.08

22'398

21'653

{PMF}

{Mi, PMF}

aldA

RecName: Full=Lactaldehyde dehydrogenase; EC=1.2.1.22; AltName: Full=Aldehyde dehydrogenase A; AltName: Full=Glycolaldehyde dehydrogenase; EC=1.2.1.21;

P25553
(ALDA_ECOLI)

2D-00153A

2D-001508

5.53

5.29

38'941

42'560

{PMF}

argG

RecName: Full=Argininosuccinate synthase; EC=6.3.4.5; AltName: Full=Citrulline--aspartate ligase;

P0A6E4
(ASSY_ECOLI)

2D-0014XO

5.28

46'220

{PMF}

argT

RecName: Full=Lysine-arginine-ornithine-binding periplasmic protein; Short=LAO-binding protein; Flags: Precursor;

P09551
(ARGT_ECOLI)

2D-0015AC

5.17

26'913

{Mi,PMF}

aroD

RecName: Full=3-dehydroquinate dehydratase; Short=3-dehydroquinase; EC=4.2.1.10; AltName: Full=Type I DHQase;

P05194
(AROD_ECOLI)

2D-0015A3

5.31

27'372

{PMF}

asnS

RecName: Full=Asparaginyl-tRNA synthetase; EC=6.1.1.22; AltName: Full=Asparagine--tRNA ligase; Short=AsnRS;

P0A8M0
(SYN_ECOLI)

2D-001533

5.01

39'019

{PMF}

atpC

RecName: Full=ATP synthase epsilon chain; AltName: Full=ATP synthase F1 sector epsilon subunit; AltName: Full=F-ATPase epsilon subunit;

P0A6E6
(ATPE_ECOLI)

2D-0015GA

5.34

10'459

{PMF}

atpD

RecName: Full=ATP synthase subunit beta; EC=3.6.3.14; AltName: Full=ATP synthase F1 sector subunit beta; AltName: Full=F-ATPase subunit beta;

P0ABB4
(ATPB_ECOLI)

2D-0014ZV

2D-0014XV

4.96

5.00

42'905

45'494

{Mi,PMF}

clpS

RecName: Full=ATP-dependent Clp protease adapter protein ClpS;

P0A8Q6
(CLPS_ECOLI)

2D-0015G6

5.40

10'724

{PMF}

crr

RecName: Full=Glucose-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=EIIA-Glc; AltName: Full=EIII-Glc; AltName: Full=PTS system glucose-specific EIIA component;

P69783
(PTGA_ECOLI)

2D-0015DT

4.94

18'465

{Mi}

cysK

RecName: Full=Cysteine synthase A; Short=CSase A; EC=2.5.1.47; AltName: Full=O-acetylserine (thiol)-lyase A; Short=OAS-TL A; AltName: Full=O-acetylserine sulfhydrylase A; AltName: Full=Sulfate starvation-induced protein 5; Short=SSI5;

4.95

5.32

5.06

5.04

28'177

32'863

29'467

28'314

{PMF}

{Mi, PMF}

{PMF}

cysM

RecName: Full=Cysteine synthase B; Short=CSase B; EC=2.5.1.47; AltName: Full=O-acetylserine (thiol)-lyase B; Short=OAS-TL B; AltName: Full=O-acetylserine sulfhydrylase B;

P16703
(CYSM_ECOLI)

2D-001HEM

5.47

31'435

{PMF}

dnaK

RecName: Full=Chaperone protein DnaK; AltName: Full=HSP70; AltName: Full=Heat shock 70 kDa protein; AltName: Full=Heat shock protein 70;

4.95

4.96

4.97

53'154

60'487

60'010

{PMF}

{Mi, PMF}

{PMF}

engD

RecName: Full=GTP-dependent nucleic acid-binding protein engD;

P0ABU2
(ENGD_ECOLI)

2D-001505

5.02

42'560

{PMF}

eno

RecName: Full=Enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=2-phosphoglycerate dehydratase;

5.16

5.46

5.20

31'562

45'494

40'139

{PMF}

{Mi, PMF}

{PMF}

fabD

RecName: Full=Malonyl CoA-acyl carrier protein transacylase; Short=MCT; EC=2.3.1.39;

P0AAI9
(FABD_ECOLI)

2D-001573

5.03

32'271

{PMF}

fliD

RecName: Full=Flagellar hook-associated protein 2; Short=HAP2; AltName: Full=Filament cap protein; AltName: Full=Flagellar cap protein;

P24216
(FLID_ECOLI)

2D-0014ZV

4.96

42'905

{Mi}

fliY

RecName: Full=Cystine-binding periplasmic protein; Short=CBP; AltName: Full=Protein fliY; AltName: Full=Sulfate starvation-induced protein 7; Short=SSI7; Flags: Precursor;

P0AEM9
(FLIY_ECOLI)

2D-00159C

2D-0015AN

5.23

5.19

28'658

26'525

{Mi}

{PMF}

fusA

RecName: Full=Elongation factor G; Short=EF-G;

P0A6M8
(EFG_ECOLI)

2D-0014R2

5.21

91'354

{PMF}

gapA

RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A; Short=GAPDH-A; EC=1.2.1.12;

P0A9B2
(G3P1_ECOLI)

2D-0015HJ

2D-0015G5

5.32

5.03

9'173

10'690

{Mi}

gpmI

RecName: Full=2,3-bisphosphoglycerate-independent phosphoglycerate mutase; Short=BPG-independent PGAM; Short=Phosphoglyceromutase; Short=iPGM; EC=5.4.2.1;

P37689
(GPMI_ECOLI)

2D-0014VM

5.15

52'457

{PMF}

gpt

RecName: Full=Xanthine phosphoribosyltransferase; EC=2.4.2.22; AltName: Full=Xanthine-guanine phosphoribosyltransferase; Short=XGPRT;

P0A9M5
(XGPT_ECOLI)

2D-0015EY

5.44

14'028

{PMF}

grcA

RecName: Full=Autonomous glycyl radical cofactor;

5.23

5.09

5.05

5.21

9'795

11'594

9'918

9'583

{PMF}

{Mi}

groL

RecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName: Full=Protein Cpn60;

P0A6F5
(CH60_ECOLI)

2D-0014V3

2D-0014UQ

4.96

4.99

54'287

55'445

{Mi, PMF}

{PMF}

groS

RecName: Full=10 kDa chaperonin; AltName: Full=GroES protein; AltName: Full=Protein Cpn10;

P0A6F9
(CH10_ECOLI)

2D-0015FD

5.23

13'055

{PMF}

grxD

RecName: Full=Glutaredoxin-4; Short=Grx4; AltName: Full=Monothiol glutaredoxin;

P0AC69
(GLRX4_ECOLI)

2D-0015GQ

4.96

10'012

{PMF}

hdeB

RecName: Full=Protein hdeB; AltName: Full=10K-L protein; Flags: Precursor;

P0AET2
(HDEB_ECOLI)

2D-0015H5

5.00

9'673

{Mi}

hisJ

RecName: Full=Histidine-binding periplasmic protein; Short=HBP; Flags: Precursor;

P0AEU0
(HISJ_ECOLI)

2D-00159H

5.14

28'451

{Mi,PMF}

hpt

RecName: Full=Hypoxanthine phosphoribosyltransferase; Short=HPRT; EC=2.4.2.8;

P0A9M2
(HPRT_ECOLI)

2D-0015FG

5.38

12'380

{PMF}

icd

RecName: Full=Isocitrate dehydrogenase [NADP]; Short=IDH; EC=1.1.1.42; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase;

P08200
(IDH_ECOLI)

2D-0014YW

5.15

43'958

{PMF}

ilvB

RecName: Full=Acetolactate synthase isozyme 1 large subunit; Short=AHAS-I; EC=2.2.1.6; AltName: Full=Acetohydroxy-acid synthase I large subunit; Short=ALS-I;

P08142
(ILVB_ECOLI)

2D-0014UM

5.22

56'329

{PMF}

katG

RecName: Full=Catalase-peroxidase; Short=CP; EC=1.11.1.21; AltName: Full=Hydroperoxidase I; Short=HPI; AltName: Full=Peroxidase/catalase;

P13029
(KATG_ECOLI)

2D-0014RB

5.16

88'816

{PMF}

livJ

RecName: Full=Leu/Ile/Val-binding protein; Short=LIV-BP; Flags: Precursor;

P0AD96
(LIVJ_ECOLI)

2D-0015E7

2D-00151X

5.21

5.31

16'972

40'301

{Mi,PMF}

livK

RecName: Full=Leucine-specific-binding protein; Short=L-BP; Short=LS-BP; Flags: Precursor;

P04816
(LIVK_ECOLI)

2D-00158X

2D-00152C

5.56

5.08

29'053

39'816

{PMF}

{Mi, PMF}

luxS

RecName: Full=S-ribosylhomocysteine lyase; EC=4.4.1.21; AltName: Full=AI-2 synthesis protein; AltName: Full=Autoinducer-2 production protein LuxS;

P45578
(LUXS_ECOLI)

2D-0015DS

2D-0015DR

5.07

5.20

18'639

18'697

{PMF}

{Mi, PMF}

malE

RecName: Full=Maltose-binding periplasmic protein; AltName: Full=MBP; AltName: Full=MMBP; AltName: Full=Maltodextrin-binding protein; Flags: Precursor;

P0AEX9
(MALE_ECOLI)

2D-00152X

2D-00153H

5.22

5.24

39'098

38'549

{Mi, PMF}

{Mi}

manX

RecName: Full=PTS system mannose-specific EIIAB component; AltName: Full=EIIAB-Man; Includes: RecName: Full=Mannose-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=EIII-Man; AltName: Full=PTS system mannose-specific EIIA component; Includes: RecName: Full=Mannose-specific phosphotransferase enzyme IIB component; EC=2.7.1.69; AltName: Full=PTS system mannose-specific EIIB component;

P69797
(PTNAB_ECOLI)

2D-0015AV

5.17

26'207

{PMF}

metK

RecName: Full=S-adenosylmethionine synthase; Short=AdoMet synthase; EC=2.5.1.6; AltName: Full=MAT; AltName: Full=Methionine adenosyltransferase;

P0A817
(METK_ECOLI)

2D-00158A

2D-0014ZS

5.00

5.13

30'312

43'079

{PMF}

mglB

RecName: Full=D-galactose-binding periplasmic protein; Short=GBP; AltName: Full=D-galactose/ D-glucose-binding protein; Short=GGBP; Flags: Precursor;

P0AEE5
(DGAL_ECOLI)

2D-00157N

5.19

31'245

{PMF}

minD

RecName: Full=Septum site-determining protein MinD; AltName: Full=Cell division inhibitor MinD;

P0AEZ3
(MIND_ECOLI)

2D-00158K

5.28

29'766

{PMF}

nfnB

RecName: Full=Oxygen-insensitive NAD(P)H nitroreductase; EC=1.-.-.-; AltName: Full=Dihydropteridine reductase; EC=1.5.1.34; AltName: Full=FMN-dependent nitroreductase;

P38489
(NFNB_ECOLI)

2D-0015EU

2D-001HEN

5.09

5.55

14'160

25'092

{PMF}

pgk

RecName: Full=Phosphoglycerate kinase; EC=2.7.2.3;

P0A799
(PGK_ECOLI)

2D-00152G

2D-00151K

5.14

5.15

39'816

40'464

{Mi}

{Mi, PMF}

pnp

RecName: Full=Polyribonucleotide nucleotidyltransferase; EC=2.7.7.8; AltName: Full=Polynucleotide phosphorylase; Short=PNPase;

P05055
(PNP_ECOLI)

2D-0014RF

5.13

87'327

{PMF}

potD

RecName: Full=Spermidine/putrescine-binding periplasmic protein; Short=SPBP; Flags: Precursor;

P0AFK9
(POTD_ECOLI)

2D-00155G

4.96

35'058

{Mi,PMF}

ppa

RecName: Full=Inorganic pyrophosphatase; EC=3.6.1.1; AltName: Full=Pyrophosphate phospho-hydrolase; Short=PPase;

P0A7A9
(IPYR_ECOLI)

2D-0015D7

5.06

21'653

{Mi,PMF}

proS

RecName: Full=Prolyl-tRNA synthetase; EC=6.1.1.15; AltName: Full=Global RNA synthesis factor; AltName: Full=Proline--tRNA ligase; Short=ProRS;

P16659
(SYP_ECOLI)

2D-0014US

5.06

55'738

{PMF}

ribH

RecName: Full=6,7-dimethyl-8-ribityllumazine synthase; Short=DMRL synthase; Short=Lumazine synthase; EC=2.5.1.9; AltName: Full=Riboflavin synthase beta chain;

P61714
(RISB_ECOLI)

2D-0015FE

5.19

12'934

{Mi,PMF}

rplI

RecName: Full=50S ribosomal protein L9;

P0A7R1
(RL9_ECOLI)

2D-0015HM

5.10

8'919

{PMF}

rpoA

RecName: Full=DNA-directed RNA polymerase subunit alpha; Short=RNAP subunit alpha; EC=2.7.7.6; AltName: Full=RNA polymerase subunit alpha; AltName: Full=Transcriptase subunit alpha;

P0A7Z4
(RPOA_ECOLI)

2D-00152B

2D-001527

5.06

5.03

39'655

39'816

{PMF}

rpsA

RecName: Full=30S ribosomal protein S1;

P0AG67
(RS1_ECOLI)

2D-0014SJ

2D-00150A

4.99

4.98

64'780

42'474

{PMF}

serC

RecName: Full=Phosphoserine aminotransferase; EC=2.6.1.52; AltName: Full=Phosphohydroxythreonine aminotransferase; Short=PSAT;

P23721
(SERC_ECOLI)

2D-00152W

2D-00156Y

5.47

5.18

39'256

32'731

{PMF}

talB

RecName: Full=Transaldolase B; EC=2.2.1.2;

P0A870
(TALB_ECOLI)

2D-00155M

5.08

34'775

{PMF}

tig

RecName: Full=Trigger factor; Short=TF;

4.97

4.93

4.95

48'212

48'981

47'081

{PMF}

tpx

RecName: Full=Thiol peroxidase; EC=1.11.1.-; AltName: Full=Scavengase P20;

P0A862
(TPX_ECOLI)

2D-0015DZ

5.00

17'510

{PMF}

trpA

RecName: Full=Tryptophan synthase alpha chain; EC=4.2.1.20;

P0A877
(TRPA_ECOLI)

2D-00159R

2D-001598

5.37

5.38

27'906

28'936

{PMF}

trxA

RecName: Full=Thioredoxin-1; Short=Trx-1;

P0AA25
(THIO_ECOLI)

2D-0015H6

4.95

9'553

{PMF}

tsf

RecName: Full=Elongation factor Ts; Short=EF-Ts;

P0A6P1
(EFTS_ECOLI)

2D-00156V

5.22

32'533

{PMF}

tufB

RecName: Full=Elongation factor Tu 2; Short=EF-Tu 2; AltName: Full=P-43;

P0CE48
(EFTU2_ECOLI)

2D-0014Y6

2D-00158F

5.58

5.14

45'311

30'129

{PMF}

uspA

RecName: Full=Universal stress protein A;

P0AED0
(USPA_ECOLI)

2D-0015FP

5.20

11'704

{Mi,PMF}

yceI

RecName: Full=Protein yceI; Flags: Precursor;

P0A8X2
(YCEI_ECOLI)

2D-0015DI

5.20

19'716

{Mi}

yciI

RecName: Full=Protein yciI;

P0AB55
(YCII_ECOLI)

2D-0015H8

5.29

9'553

{PMF}

znuA

RecName: Full=High-affinity zinc uptake system protein znuA; Flags: Precursor;

P39172
(ZNUA_ECOLI)

2D-00155Y

5.58

34'287

{PMF}

Mapping legends: { Mi } => 'Microsequencing', { PMF } => 'Peptide mass fingerprinting'

Cited references
#	PubMed/Medline	Authors	Location
[1]	11680886	Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.;	Proteomics 1(1):409-423(2001). ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';

Gel experimental information
Information Type	Data
Map name	ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Dimension	2-D
Related documents	Sample Preparation / Gel Related Documents
Strain Comment [species]	W3110 [Escherichia coli / TaxID:562]
pI	start: 4.50 - end: 5.50
Mw	start: 10000 - end: 200000
Number of detected spots	1027
Number of identified spots	97
Number of identified proteins	65
Reference	Bibliographic reference
Graphical interface

SWISS-2DPAGE (search Map)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

Attention: World-2DPAGE is no longer maintained.

Protein list for SWISS-2DPAGE ECOLI4.5-5.5 Escherichia coli(4.5-5.5)

SWISS-2DPAGE (search Map)

Protein list for SWISS-2DPAGE ECOLI4.5-5.5
Escherichia coli(4.5-5.5)