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SWISS-2DPAGE 
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Protein list for SWISS-2DPAGE CEC_HUMAN 
Colorectal epithelia cells  {Homo sapiens (Human)}
tissue: Colon epithelium


(map ID: b07dde62-03ed-4d5a-b300-a399bafec27f)
Release: 19.2 -- 09 November 2011

Sample Preparation / Gel Related Documents


Protein list for cec_human   56 identified spots / 40 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods Comment Topics References
ACTB RecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed; P60709
(ACTB_HUMAN)
2D-000TWW
2D-000TWX
5.05
5.11
41'700
41'785
{Gm}
{Gm}
-
-
1
1
ATP5B RecName: Full=ATP synthase subunit beta, mitochondrial; EC=3.6.3.14; Flags: Precursor; P06576
(ATPB_HUMAN)
2D-000TKM
4.93
49'174
{Gm}
-
1
ATP5D RecName: Full=ATP synthase subunit delta, mitochondrial; AltName: Full=F-ATPase delta subunit; Flags: Precursor; P30049
(ATPD_HUMAN)
2D-000UXY
4.50
13'360
{Gm}
-
1
ATP5J RecName: Full=ATP synthase-coupling factor 6, mitochondrial; Short=ATPase subunit F6; Flags: Precursor; P18859
(ATP5J_HUMAN)
2D-000V17
5.31
11'038
{Mi}
-
1
B2M RecName: Full=Beta-2-microglobulin; Contains: RecName: Full=Beta-2-microglobulin form pI 5.3; Flags: Precursor; P61769
(B2MG_HUMAN)
2D-000V14
6.40
10'997
{Mi}
-
1
CALR RecName: Full=Calreticulin; AltName: Full=CRP55; AltName: Full=Calregulin; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ERp60; AltName: Full=HACBP; AltName: Full=grp60; Flags: Precursor; P27797
(CALR_HUMAN)
2D-000TB6
4.40
58'700
{Gm}
-
1
CAT RecName: Full=Catalase; EC=1.11.1.6; P04040
(CATA_HUMAN)
2D-000TCL
2D-000TCT
2D-000TCE
6.75
7.07
6.51
57'870
57'752
57'870
{Gm}
{Gm}
{Gm}
-
-
-
1
1
1
CD44 RecName: Full=CD44 antigen; AltName: Full=CDw44; AltName: Full=Epican; AltName: Full=Extracellular matrix receptor III; Short=ECMR-III; AltName: Full=GP90 lymphocyte homing/adhesion receptor; AltName: Full=HUTCH-I; AltName: Full=Heparan sulfate proteoglycan; AltName: Full=Hermes antigen; AltName: Full=Hyaluronate receptor; AltName: Full=Phagocytic glycoprotein 1; Short=PGP-1; AltName: Full=Phagocytic glycoprotein I; Short=PGP-I; AltName: CD_antigen=CD44; Flags: Precursor; P16070
(CD44_HUMAN)
2D-000TTU
2D-0010AT
2D-0010AS
5.06
5.11
5.23
43'699
42'731
43'168
{Im}
{Im}
{Im}
Expression
Expression
Expression
1, 2
1, 2
1, 2
CMPK1 RecName: Full=UMP-CMP kinase; EC=2.7.4.14; AltName: Full=Cytidine monophosphate kinase; AltName: Full=Cytidylate kinase; AltName: Full=Deoxycytidylate kinase; AltName: Full=Uridine monophosphate kinase; AltName: Full=Uridine monophosphate/cytidine monophosphate kinase; Short=UMP/CMP kinase; Short=UMP/CMPK; P30085
(KCY_HUMAN)
2D-000UQW
5.42
21'422
{Mi,Gm}
-
1
COX5A RecName: Full=Cytochrome c oxidase subunit 5A, mitochondrial; AltName: Full=Cytochrome c oxidase polypeptide Va; Flags: Precursor; P20674
(COX5A_HUMAN)
2D-000UZ5
4.80
12'121
{Gm}
-
1
COX5B RecName: Full=Cytochrome c oxidase subunit 5B, mitochondrial; AltName: Full=Cytochrome c oxidase polypeptide Vb; Flags: Precursor; P10606
(COX5B_HUMAN)
2D-000UZJ
5.62
12'121
{Gm}
-
1
CRYAB RecName: Full=Alpha-crystallin B chain; AltName: Full=Alpha(B)-crystallin; AltName: Full=Heat shock protein beta-5; Short=HspB5; AltName: Full=Renal carcinoma antigen NY-REN-27; AltName: Full=Rosenthal fiber component; P02511
(CRYAB_HUMAN)
2D-000UUK
8.52
16'874
{Gm}
-
1
CTSD RecName: Full=Cathepsin D; EC=3.4.23.5; Contains: RecName: Full=Cathepsin D light chain; Contains: RecName: Full=Cathepsin D heavy chain; Flags: Precursor; P07339
(CATD_HUMAN)
2D-000UEX
2D-000UGF
5.56
5.40
28'114
27'363
{Mi}
{Mi}
-
-
1
1
ECHS1 RecName: Full=Enoyl-CoA hydratase, mitochondrial; EC=4.2.1.17; AltName: Full=Enoyl-CoA hydratase 1; AltName: Full=Short-chain enoyl-CoA hydratase; Short=SCEH; Flags: Precursor; P30084
(ECHM_HUMAN)
2D-000UJX
5.95
25'821
{Mi}
-
1
EEF1B2 RecName: Full=Elongation factor 1-beta; Short=EF-1-beta; P24534
(EF1B_HUMAN)
2D-000UCG
4.54
30'421
{Gm}
-
1
EGFR RecName: Full=Epidermal growth factor receptor; EC=2.7.10.1; AltName: Full=Proto-oncogene c-ErbB-1; AltName: Full=Receptor tyrosine-protein kinase erbB-1; Flags: Precursor; P00533
(EGFR_HUMAN)
2D-0010AO
2D-0010AP
2D-0010AN
5.93
5.73
5.85
151'853
150'458
151'853
{Im}
{Im}
{Im}
-
-
-
1
1
1
ERP29 RecName: Full=Endoplasmic reticulum resident protein 29; Short=ERp29; AltName: Full=Endoplasmic reticulum resident protein 28; Short=ERp28; AltName: Full=Endoplasmic reticulum resident protein 31; Short=ERp31; Flags: Precursor; P30040
(ERP29_HUMAN)
2D-000UID
6.14
26'565
{Mi}
-
1
GLUD1 RecName: Full=Glutamate dehydrogenase 1, mitochondrial; Short=GDH 1; EC=1.4.1.3; Flags: Precursor; P00367
(DHE3_HUMAN)
2D-000TH6
2D-000THK
2D-000THF
7.28
6.92
6.51
53'020
52'912
53'020
{Gm}
{Gm}
{Gm}
-
-
-
1
1
1
GSTP1 RecName: Full=Glutathione S-transferase P; EC=2.5.1.18; AltName: Full=GST class-pi; AltName: Full=GSTP1-1; P09211
(GSTP1_HUMAN)
2D-000UP8
5.46
22'917
{Gm}
-
1
HBA1 RecName: Full=Hemoglobin subunit alpha; AltName: Full=Alpha-globin; AltName: Full=Hemoglobin alpha chain; P69905
(HBA_HUMAN)
2D-000V0N
9.44
11'289
{Gm}
-
1
HBB RecName: Full=Hemoglobin subunit beta; AltName: Full=Beta-globin; AltName: Full=Hemoglobin beta chain; Contains: RecName: Full=LVV-hemorphin-7; P68871
(HBB_HUMAN)
2D-000V2C
2D-000V0U
6.24
6.44
10'204
11'163
{Mi,Gm}
{Mi,Gm}
-
-
1
1
HSPA9 RecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; Short=MOT; AltName: Full=Peptide-binding protein 74; Short=PBP74; Flags: Precursor; P38646
(GRP75_HUMAN)
2D-000T66
2D-000T6C
5.40
5.32
73'173
73'173
{Mi,Gm}
{Mi,Gm}
-
-
1
1
HSPD1 RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=HuCHA60; AltName: Full=Mitochondrial matrix protein P1; AltName: Full=P60 lymphocyte protein; Flags: Precursor; P10809
(CH60_HUMAN)
2D-000TD7
2D-000TD4
2D-000TCO
5.09
5.16
5.04
56'244
56'244
56'704
{Mi}
{Mi}
{Mi}
-
-
-
1
1
1
LGALS1 RecName: Full=Galectin-1; Short=Gal-1; AltName: Full=14 kDa laminin-binding protein; Short=HLBP14; AltName: Full=14 kDa lectin; AltName: Full=Beta-galactoside-binding lectin L-14-I; AltName: Full=Galaptin; AltName: Full=HBL; AltName: Full=HPL; AltName: Full=Lactose-binding lectin 1; AltName: Full=Lectin galactoside-binding soluble 1; AltName: Full=Putative MAPK-activating protein PM12; AltName: Full=S-Lac lectin 1; P09382
(LEG1_HUMAN)
2D-000UZI
4.93
12'076
{Gm}
-
1
MRPL12 RecName: Full=39S ribosomal protein L12, mitochondrial; Short=L12mt; Short=MRP-L12; AltName: Full=5c5-2; Flags: Precursor; P52815
(RM12_HUMAN)
2D-000UU1
5.01
17'498
{Mi}
-
1
MYL1 RecName: Full=Myosin light chain 1/3, skeletal muscle isoform; Short=MLC1/MLC3; Short=MLC1F/MLC3F; AltName: Full=Myosin light chain alkali 1/2; Short=Myosin light chain A1/A2; P05976
(MYL1_HUMAN)
2D-000URH
5.06
20'819
{Gm}
-
1
NDUFA8 RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; AltName: Full=Complex I-19kD; Short=CI-19kD; AltName: Full=Complex I-PGIV; Short=CI-PGIV; AltName: Full=NADH-ubiquinone oxidoreductase 19 kDa subunit; P51970
(NDUA8_HUMAN)
2D-000UUK
8.52
16'874
{Gm}
-
1
NDUFV2 RecName: Full=NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; EC=1.6.5.3; EC=1.6.99.3; AltName: Full=NADH-ubiquinone oxidoreductase 24 kDa subunit; Flags: Precursor; P19404
(NDUV2_HUMAN)
2D-000UOL
5.85
23'156
{Mi}
-
1
PDHB RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; Short=PDHE1-B; EC=1.2.4.1; Flags: Precursor; P11177
(ODPB_HUMAN)
2D-000U6F
5.38
34'240
{Mi}
-
1
PDIA3 RecName: Full=Protein disulfide-isomerase A3; EC=5.3.4.1; AltName: Full=58 kDa glucose-regulated protein; AltName: Full=58 kDa microsomal protein; Short=p58; AltName: Full=Disulfide isomerase ER-60; AltName: Full=Endoplasmic reticulum resident protein 57; Short=ER protein 57; Short=ERp57; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ER protein 60; Short=ERp60; Flags: Precursor; P30101
(PDIA3_HUMAN)
2D-000THC
2D-000TGR
2D-000TH0
5.58
5.49
5.70
52'697
52'805
52'483
{Mi,Gm}
{Mi,Gm}
{Mi,Gm}
-
-
-
1
1
1
PMS2 RecName: Full=Mismatch repair endonuclease PMS2; EC=3.1.-.-; AltName: Full=DNA mismatch repair protein PMS2; AltName: Full=PMS1 protein homolog 2; P54278
(PMS2_HUMAN)
2D-0010AQ
6.39
52'377
{Im}
-
1
PRDX3 RecName: Full=Thioredoxin-dependent peroxide reductase, mitochondrial; EC=1.11.1.15; AltName: Full=Antioxidant protein 1; Short=AOP-1; AltName: Full=HBC189; AltName: Full=Peroxiredoxin III; Short=Prx-III; AltName: Full=Peroxiredoxin-3; AltName: Full=Protein MER5 homolog; Flags: Precursor; P30048
(PRDX3_HUMAN)
2D-000UOW
6.07
22'917
{Mi}
-
1
RPL12 RecName: Full=60S ribosomal protein L12; P30050
(RL12_HUMAN)
2D-000V17
5.31
11'038
{Mi}
-
1
SERPINE1 RecName: Full=Plasminogen activator inhibitor 1; Short=PAI; Short=PAI-1; AltName: Full=Endothelial plasminogen activator inhibitor; AltName: Full=Serpin E1; Flags: Precursor; P05121
(PAI1_HUMAN)
2D-0010AR
6.97
48'577
{Im}
-
1
SOD1 RecName: Full=Superoxide dismutase [Cu-Zn]; EC=1.15.1.1; AltName: Full=Superoxide dismutase 1; Short=hSod1; P00441
(SODC_HUMAN)
2D-000UV4
2D-000UV3
5.70
5.42
16'272
16'356
{Gm}
{Gm}
-
-
1
1
SOD2 RecName: Full=Superoxide dismutase [Mn], mitochondrial; EC=1.15.1.1; Flags: Precursor; P04179
(SODM_HUMAN)
2D-000UR5
2D-000UR6
7.37
7.78
21'201
21'256
{Im}
{Im}
-
-
1
1
SPTB RecName: Full=Spectrin beta chain, erythrocyte; AltName: Full=Beta-I spectrin; P11277
(SPTB1_HUMAN)
2D-000UKZ
5.60
25'095
{Gm}
-
1
TPI1 RecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase; P60174
(TPIS_HUMAN)
2D-000UJS
6.38
25'956
{Gm}
-
1
TPT1 RecName: Full=Translationally-controlled tumor protein; Short=TCTP; AltName: Full=Fortilin; AltName: Full=Histamine-releasing factor; Short=HRF; AltName: Full=p23; P13693
(TCTP_HUMAN)
2D-0010AU
4.75
21'091
{Im}
-
1
TXN RecName: Full=Thioredoxin; Short=Trx; AltName: Full=ATL-derived factor; Short=ADF; AltName: Full=Surface-associated sulphydryl protein; Short=SASP; P10599
(THIO_HUMAN)
2D-000V0S
4.84
11'121
{Gm}
-
1
Mapping legends: { Gm } => 'Gel matching', { Im } => 'Immunobloting', { Mi } => 'Microsequencing'



Cited references

# PubMed/Medline Authors Location
[1] 9504818 Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.; Electrophoresis 18(1):2842-2848(1997).
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';'';
[2] 7553660 Gunthert U., Stauder R., Mayer B., Terpe H.J., Finke L., Friedrichs K.; Cancer Surv. 24(1):19-42(1995).
''''''Are CD44 variant isoforms involved in human tumour progression?'';'';'';


Gel experimental information

Information Type Data
Map name CEC_HUMAN {Colorectal epithelia cells}
Dimension 2-D
Related documents Sample Preparation / Gel Related Documents
species Homo sapiens (Human) / TaxID:9606
Tissue (Swiss-Prot definition) Colon epithelium
pI start: 3.50 - end: 10.00
Mw start: 10000 - end: 200000
Number of detected spots 3077
Number of identified spots 56
Number of identified proteins 40
Reference Bibliographic reference
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SWISS-2DPAGE (search Map)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server