World-2DPAGE Home
Home  |  Contact

Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.


SWISS-2DPAGE 
Search by  [accession number] 
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] *
[graphical interface]

     Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal
World-2DPAGE Repository

Exclude local DBs
has only effect if a remote
interface is selected
     

Protein list for SWISS-2DPAGE NUCLEOLI_HELA_2D_HUMAN 
2D-PAGE of nucleolar proteins from Human HeLa cells  {Homo sapiens (Human)}
tissue: Cervix carcinoma


(map ID: b30ddfe7-c03e-4164-b5e0-5203009565bc)
Release: 19.2 -- 09 November 2011

Sample Preparation / Gel Related Documents


Protein list for nucleoli_hela_2d_human   51 identified spots / 44 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods References
ACTB RecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed; P60709
(ACTB_HUMAN)
2D-001VNR
5.47
44'313
{PMF}
1
BCAS2 RecName: Full=Pre-mRNA-splicing factor SPF27; AltName: Full=Breast carcinoma-amplified sequence 2; AltName: Full=DNA amplified in mammary carcinoma 1 protein; AltName: Full=Spliceosome-associated protein SPF 27; O75934
(SPF27_HUMAN)
2D-001VW1
5.50
29'411
{PMF}
1
CBX3 RecName: Full=Chromobox protein homolog 3; AltName: Full=HECH; AltName: Full=Heterochromatin protein 1 homolog gamma; Short=HP1 gamma; AltName: Full=Modifier 2 protein; Q13185
(CBX3_HUMAN)
2D-001W0V
5.27
22'283
{MS/MS}
1
CCT2 RecName: Full=T-complex protein 1 subunit beta; Short=TCP-1-beta; AltName: Full=CCT-beta; P78371
(TCPB_HUMAN)
2D-001VKH
6.56
55'522
{PMF}
1
CCT5 RecName: Full=T-complex protein 1 subunit epsilon; Short=TCP-1-epsilon; AltName: Full=CCT-epsilon; P48643
(TCPE_HUMAN)
2D-001VIU
5.67
61'221
{PMF}
1
EBNA1BP2 RecName: Full=Probable rRNA-processing protein EBP2; AltName: Full=EBNA1-binding protein 2; AltName: Full=Nucleolar protein p40; Q99848
(EBP2_HUMAN)
2D-001VOQ
6.59
41'615
{PMF}
1
EIF6 RecName: Full=Eukaryotic translation initiation factor 6; Short=eIF-6; AltName: Full=B(2)GCN homolog; AltName: Full=B4 integrin interactor; AltName: Full=CAB; AltName: Full=p27(BBP); P56537
(IF6_HUMAN)
2D-001VTA
5.94
33'604
{MS/MS}
1
EXOSC4 RecName: Full=Exosome complex component RRP41; AltName: Full=Exosome component 4; AltName: Full=Ribosomal RNA-processing protein 41; AltName: Full=p12A; Q9NPD3
(EXOS4_HUMAN)
2D-001VVY
6.55
29'587
{PMF}
1
GLTSCR2 RecName: Full=Glioma tumor suppressor candidate region gene 2 protein; AltName: Full=p60; Q9NZM5
(GSCR2_HUMAN)
2D-001VI0
2D-001VHW
6.34
6.47
64'287
64'772
{PMF}
{PMF}
1
1
GNL3 RecName: Full=Guanine nucleotide-binding protein-like 3; AltName: Full=E2-induced gene 3 protein; AltName: Full=Novel nucleolar protein 47; Short=NNP47; AltName: Full=Nucleolar GTP-binding protein 3; AltName: Full=Nucleostemin; Q9BVP2
(GNL3_HUMAN)
2D-001VHW
6.47
64'772
{PMF,MS/MS}
1
GRWD1 RecName: Full=Glutamate-rich WD repeat-containing protein 1; Q9BQ67
(GRWD1_HUMAN)
2D-001VKA
4.97
56'151
{PMF}
1
HIST1H4A RecName: Full=Histone H4; P62805
(H4_HUMAN)
2D-001W7A
6.28
15'233
{PMF}
1
HNRNPK RecName: Full=Heterogeneous nuclear ribonucleoprotein K; Short=hnRNP K; AltName: Full=Transformation up-regulated nuclear protein; Short=TUNP; P61978
(HNRPK_HUMAN)
2D-001VI9
2D-001VII
2D-001VIA
2D-001VIB
5.50
5.43
5.52
5.61
63'566
61'915
63'805
63'805
{PMF}
{PMF}
{PMF}
{MS/MS}
1
1
1
1
HSPA1A RecName: Full=Heat shock 70 kDa protein 1A/1B; AltName: Full=Heat shock 70 kDa protein 1/2; Short=HSP70-1/HSP70-2; Short=HSP70.1/HSP70.2; P08107
(HSP71_HUMAN)
2D-001VH5
5.65
68'886
{PMF}
1
HSPA8 RecName: Full=Heat shock cognate 71 kDa protein; AltName: Full=Heat shock 70 kDa protein 8; P11142
(HSP7C_HUMAN)
2D-001VGX
5.50
70'432
{PMF,MS/MS}
1
HSPA9 RecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; Short=MOT; AltName: Full=Peptide-binding protein 74; Short=PBP74; Flags: Precursor; P38646
(GRP75_HUMAN)
2D-001VGG
5.69
72'414
{PMF}
1
HSPD1 RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=HuCHA60; AltName: Full=Mitochondrial matrix protein P1; AltName: Full=P60 lymphocyte protein; Flags: Precursor; P10809
(CH60_HUMAN)
2D-001VIT
5.50
60'991
{PMF}
1
KRT10 RecName: Full=Keratin, type I cytoskeletal 10; AltName: Full=Cytokeratin-10; Short=CK-10; AltName: Full=Keratin-10; Short=K10; P13645
(K1C10_HUMAN)
2D-001VL8
6.19
52'478
{PMF}
1
KRT17 RecName: Full=Keratin, type I cytoskeletal 17; AltName: Full=39.1; AltName: Full=Cytokeratin-17; Short=CK-17; AltName: Full=Keratin-17; Short=K17; Q04695
(K1C17_HUMAN)
2D-001VMG
5.19
48'313
{MS/MS}
1
KRT18 RecName: Full=Keratin, type I cytoskeletal 18; AltName: Full=Cell proliferation-inducing gene 46 protein; AltName: Full=Cytokeratin-18; Short=CK-18; AltName: Full=Keratin-18; Short=K18; P05783
(K1C18_HUMAN)
2D-001VND
5.59
45'837
{PMF}
1
KRT1 RecName: Full=Keratin, type II cytoskeletal 1; AltName: Full=67 kDa cytokeratin; AltName: Full=Cytokeratin-1; Short=CK-1; AltName: Full=Hair alpha protein; AltName: Full=Keratin-1; Short=K1; AltName: Full=Type-II keratin Kb1; P04264
(K2C1_HUMAN)
2D-001VK9
4.92
56'151
{PMF}
1
KRT7 RecName: Full=Keratin, type II cytoskeletal 7; AltName: Full=Cytokeratin-7; Short=CK-7; AltName: Full=Keratin-7; Short=K7; AltName: Full=Sarcolectin; AltName: Full=Type-II keratin Kb7; P08729
(K2C7_HUMAN)
2D-001VKG
5.63
56'151
{PMF,MS/MS}
1
KRT8 RecName: Full=Keratin, type II cytoskeletal 8; AltName: Full=Cytokeratin-8; Short=CK-8; AltName: Full=Keratin-8; Short=K8; AltName: Full=Type-II keratin Kb8; P05787
(K2C8_HUMAN)
2D-001VKW
5.86
53'474
{PMF,MS/MS}
1
LMNA RecName: Full=Prelamin-A/C; Contains: RecName: Full=Lamin-A/C; AltName: Full=70 kDa lamin; AltName: Full=Renal carcinoma antigen NY-REN-32; Flags: Precursor; P02545
(LMNA_HUMAN)
2D-001VI5
2D-001VG3
6.68
6.76
64'287
75'283
{PMF}
{PMF}
1
1
NOP16 RecName: Full=Nucleolar protein 16; AltName: Full=HBV pre-S2 trans-regulated protein 3; Q9Y3C1
(NOP16_HUMAN)
2D-001VZL
6.45
23'931
{PMF}
1
NPM1 RecName: Full=Nucleophosmin; Short=NPM; AltName: Full=Nucleolar phosphoprotein B23; AltName: Full=Nucleolar protein NO38; AltName: Full=Numatrin; P06748
(NPM_HUMAN)
2D-001VRG
2D-001VPE
2D-001VR9
2D-001VQC
4.65
4.79
4.79
4.68
36'910
38'894
37'314
38'388
{PMF}
{PMF}
{MS/MS, PMF}
{PMF}
1
1
1
1
PA2G4 RecName: Full=Proliferation-associated protein 2G4; AltName: Full=Cell cycle protein p38-2G4 homolog; Short=hG4-1; AltName: Full=ErbB3-binding protein 1; Q9UQ80
(PA2G4_HUMAN)
2D-001VN0
2D-001VMW
6.64
6.43
47'059
47'236
{PMF}
{PMF}
1
1
PDIA3 RecName: Full=Protein disulfide-isomerase A3; EC=5.3.4.1; AltName: Full=58 kDa glucose-regulated protein; AltName: Full=58 kDa microsomal protein; Short=p58; AltName: Full=Disulfide isomerase ER-60; AltName: Full=Endoplasmic reticulum resident protein 57; Short=ER protein 57; Short=ERp57; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ER protein 60; Short=ERp60; Flags: Precursor; P30101
(PDIA3_HUMAN)
2D-001VJN
6.00
57'865
{PMF,MS/MS}
1
PKNOX1 RecName: Full=Homeobox protein PKNOX1; AltName: Full=Homeobox protein PREP-1; AltName: Full=PBX/knotted homeobox 1; P55347
(PKNX1_HUMAN)
2D-001VJN
6.00
57'865
{MS/MS}
1
POLR1C RecName: Full=DNA-directed RNA polymerases I and III subunit RPAC1; Short=DNA-directed RNA polymerase I subunit C; Short=RNA polymerases I and III subunit AC1; AltName: Full=AC40; AltName: Full=DNA-directed RNA polymerases I and III 40 kDa polypeptide; Short=RPA40; AltName: Full=RPA39; AltName: Full=RPC40; O15160
(RPAC1_HUMAN)
2D-001VOK
5.50
42'256
{PMF}
1
POLR1D RecName: Full=DNA-directed RNA polymerases I and III subunit RPAC2; Short=RNA polymerases I and III subunit AC2; AltName: Full=AC19; AltName: Full=DNA-directed RNA polymerase I subunit D; AltName: Full=RNA polymerase I 16 kDa subunit; Short=RPA16; AltName: Full=RPC16; AltName: Full=hRPA19; Q9Y2S0
(RPAC2_HUMAN)
2D-001W5I
5.65
17'077
{PMF}
1
POLR2E RecName: Full=DNA-directed RNA polymerases I, II, and III subunit RPABC1; Short=RNA polymerases I, II, and III subunit ABC1; AltName: Full=DNA-directed RNA polymerase II 23 kDa polypeptide; AltName: Full=DNA-directed RNA polymerase II subunit E; AltName: Full=RPB5 homolog; AltName: Full=XAP4; P19388
(RPAB1_HUMAN)
2D-001VX3
6.01
28'212
{PMF}
1
PRDX2 RecName: Full=Peroxiredoxin-2; EC=1.11.1.15; AltName: Full=Natural killer cell-enhancing factor B; Short=NKEF-B; AltName: Full=PRP; AltName: Full=Thiol-specific antioxidant protein; Short=TSA; AltName: Full=Thioredoxin peroxidase 1; AltName: Full=Thioredoxin-dependent peroxide reductase 1; P32119
(PRDX2_HUMAN)
2D-001VZP
5.70
24'026
{PMF,MS/MS}
1
PRPF19 RecName: Full=Pre-mRNA-processing factor 19; AltName: Full=Nuclear matrix protein 200; AltName: Full=PRP19/PSO4 homolog; Short=hPso4; AltName: Full=Senescence evasion factor; Q9UMS4
(PRP19_HUMAN)
2D-001VKI
6.67
55'941
{PMF}
1
RBM4B RecName: Full=RNA-binding protein 4B; AltName: Full=RNA-binding motif protein 30; AltName: Full=RNA-binding motif protein 4B; AltName: Full=RNA-binding protein 30; Q9BQ04
(RBM4B_HUMAN)
2D-001VOQ
6.59
41'615
{PMF}
1
RPL9 RecName: Full=60S ribosomal protein L9; P32969
(RL9_HUMAN)
2D-001VZ6
5.96
24'459
{PMF}
1
RRS1 RecName: Full=Ribosome biogenesis regulatory protein homolog; Q15050
(RRS1_HUMAN)
2D-001VO0
6.15
43'488
{PMF}
1
RUVBL1 RecName: Full=RuvB-like 1; EC=3.6.4.12; AltName: Full=49 kDa TATA box-binding protein-interacting protein; Short=49 kDa TBP-interacting protein; AltName: Full=54 kDa erythrocyte cytosolic protein; Short=ECP-54; AltName: Full=INO80 complex subunit H; AltName: Full=Nuclear matrix protein 238; Short=NMP 238; AltName: Full=Pontin 52; AltName: Full=TIP49a; AltName: Full=TIP60-associated protein 54-alpha; Short=TAP54-alpha; Q9Y265
(RUVB1_HUMAN)
2D-001VKX
6.74
53'474
{PMF}
1
S100A11 RecName: Full=Protein S100-A11; AltName: Full=Calgizzarin; AltName: Full=Metastatic lymph node gene 70 protein; Short=MLN 70; AltName: Full=Protein S100-C; AltName: Full=S100 calcium-binding protein A11; P31949
(S10AB_HUMAN)
2D-001W96
6.26
13'402
{PMF}
1
S100A16 RecName: Full=Protein S100-A16; AltName: Full=Aging-associated gene 13 protein; AltName: Full=Protein S100-F; AltName: Full=S100 calcium-binding protein A16; Q96FQ6
(S10AG_HUMAN)
2D-001W8L
6.57
14'085
{PMF}
1
S100A4 RecName: Full=Protein S100-A4; AltName: Full=Calvasculin; AltName: Full=Metastasin; AltName: Full=Placental calcium-binding protein; AltName: Full=Protein Mts1; AltName: Full=S100 calcium-binding protein A4; P26447
(S10A4_HUMAN)
2D-001W9G
5.72
14'400
{PMF,MS/MS}
1
TSPYL1 RecName: Full=Testis-specific Y-encoded-like protein 1; Short=TSPY-like protein 1; Q9H0U9
(TSYL1_HUMAN)
2D-001VIK
5.56
61'915
{PMF}
1
VIM RecName: Full=Vimentin; P08670
(VIME_HUMAN)
2D-001VJV
2D-001VJW
5.21
5.27
57'216
57'216
{PMF}
{PMF}
1
1
ZNF593 RecName: Full=Zinc finger protein 593; AltName: Full=Zinc finger protein T86; O00488
(ZN593_HUMAN)
2D-001W30
5.58
19'433
{PMF}
1
Mapping legends: { MS/MS } => 'Tandem mass spectrometry', { PMF } => 'Peptide mass fingerprinting'



Cited references

# PubMed/Medline Authors Location
[1] 12429849 Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.; Mol. Biol. Cell. 13(1):4100-4109(2002).
''''''Functional proteomic analysis of human nucleolus'';'';'';


Gel experimental information

Information Type Data
Map name NUCLEOLI_HELA_2D_HUMAN {2D-PAGE of nucleolar proteins from Human HeLa cells}
Dimension 2-D
Related documents Sample Preparation / Gel Related Documents
species Homo sapiens (Human) / TaxID:9606
Tissue (Swiss-Prot definition) Cervix carcinoma
pI start: 3.50 - end: 10.00
Mw start: 10000 - end: 200000
Number of detected spots 1271
Number of identified spots 51
Number of identified proteins 44
Reference Bibliographic reference
Graphical interface
[Click for graphical interface]


SWISS-2DPAGE image

SWISS-2DPAGE (search Map)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server